Journal: Antioxidants (Basel, Switzerland)

Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.

doi: 10.3390/antiox11101881

Figure Lengend Snippet: Figure 9. Effects of UF and LCF on Nrf2/Keap1 signaling pathway. Effects of UF and LCF on Nrf2 content (a) and mRNA expression (b), and Keap1 content (c) and mRNA expression (d). Effects of UF and LCF on activity of NQO-1 (e) and mRNA expression (f), HO-1 content (g) and mRNA expression (h), and GCLC content (i) and mRNA expression (j). UF: unfermentation; LCF: single microbial fermentation with L. curvatus. Analysis of significant differences between each group and H2O2 model group, #: p < 0.05; ##: p < 0.01; ###: p < 0.001.; comparison of each group between UF and LCF, *: p < 0.05; **: p < 0.01; ***: p < 0.001; ns: p > 0.05.

Article Snippet: PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China.

Techniques: Expressing, Activity Assay, Comparison

Journal: Antioxidants (Basel, Switzerland)

Article Title: Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H 2 O 2 -Induced Oxidative Stress in Human Skin Fibroblasts.

doi: 10.3390/antiox11101881

Figure Lengend Snippet: Figure 13. The oxidative stress pathway of Nrf2/Keap1/Mafk.

Article Snippet: PBS, DMEM medium, newborn calf serum, streptomycin (100 mg/L), penicillin (1 × 105 U/L), and 0.25% trypsin (including EDTA) were purchased from GIBCO Life Technologies, Shanghai, China; ascorbic acid and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Alfa Aesar, China; Chemical Co., Ltd., Shanghai, China; Western and IP cell lysate, PMSF and ROS Detection Kits were purchased from Biyuntian Biotechnology Co., Ltd., Shanghai, China; CCK-8 Cell Proliferation and Toxicity Detection Kit (Biorigin (Beijing) Inc., Beijing, China; Total Glutathione Peroxidase (GSH-Px) Assay Kit with Nicotinamide Adenine Dinucleotide Phosphate (NADPH), Catalase Assay Kit, ROS Detection Kit and Cell Counting Kit, Total Antioxidant Capacity Test Kit (ABTS method) were purchased from Beyotime Biotechnology Co., Ltd., Shanghai, China; Human Elastase ELISA Kit, Human Collagen Type I ELISA Kit, Human Total Matrix Metalloproteinase 1 (MMP-1) ELISA Kit and Human NRF2 ELISA Kit were purchased from Cusabio (https://www.cusabio.cn/, accessed on 23 March 2022), Wuhan, China.

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Self-immolative nanocapsules precisely regulate depressive neuronal microenvironment for synergistic antidepression therapy

doi: 10.1186/s12951-023-02008-9

Figure Lengend Snippet: In vivo antidepressive effects of nanocapsules. A In vivo Cy7.5 fluorescence of C57BL/6J mice and CUMS mice after intravenous injection with VCNCs-Cy7.5, CNCs-Cy7.5, and VNCs-Cy7.5 at 400 μg 5-HT/kg for 3 h and 7 h. Living Cy 5.5 fluorescence images of mice after treatment with VCNCs-Cy5.5, CNCs-Cy5.5, and VNCs -Cy5.5 (C 5-HT : 400 μg /kg) for 3 h. The representative western blot analysis of B Nrf 2 in the hippocampus of brain across all groups. The other two replicates were presented in Additional file : Fig. S38. Reverse transcription quantitative polymerase chain reaction analysis (RT-qPCR) of relative mRNA levels of C NLRP3 in the hippocampi of mice in all groups (n = 3). The levels of hippocampal D IL-6 in the mice hippocampi after treatment were detected using ELISA kits. The results were normalized by the protein concentration of each sample (n = 3). E ROS/DAPI staining and F GFAP / DAPI staining brains in groups subjected to different treatments. Analysis of hippocampal BDNF expression using G western blot, H RT-qPCR, I ELISA kits and J immunohistochemistry slides. The significant differences between groups were analyzed using the one-way ANOVA method, *P < 0.05, **P < 0.01, *** P < 0.001

Article Snippet: The sample solutions were then measured using IL-6 (Elabscience, #E-MSEL-M0001, China), IL-1β (Elabscience, #E-EL-M0037c, China), TNF-α (Elabscience, #E-EL-M3063, China), BDNF (Elabscience, #E-EL-M0203c, China), Nrf2 (Cusabio, #CSB-E16188m, China), and 5-HT ELISA kits (Elabscience, #E-EL-0033c China) according to the manufacturer’s instructions.

Techniques: In Vivo, Fluorescence, Injection, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Protein Concentration, Staining, Expressing, Immunohistochemistry

Journal: Journal of Nanobiotechnology

Article Title: Self-immolative nanocapsules precisely regulate depressive neuronal microenvironment for synergistic antidepression therapy

doi: 10.1186/s12951-023-02008-9

Figure Lengend Snippet: Effects of nanocapsules on in vivo 5-HT level and neuroprotection. A 5-HT IHC and B nissil and HE staining of brains after administering with fluoxetine, VCNCs, CNCs, and VNCs. C The levels of hippocampal 5-HT in the mice hippocampi after treatment were detected using ELISA kits. The results were normalized by the protein concentration of each sample (n = 3). The significant differences between groups were analyzed using the one-way ANOVA method, *P < 0.05. D The in vivo therapeutic outcomes of nanocapsules

Article Snippet: The sample solutions were then measured using IL-6 (Elabscience, #E-MSEL-M0001, China), IL-1β (Elabscience, #E-EL-M0037c, China), TNF-α (Elabscience, #E-EL-M3063, China), BDNF (Elabscience, #E-EL-M0203c, China), Nrf2 (Cusabio, #CSB-E16188m, China), and 5-HT ELISA kits (Elabscience, #E-EL-0033c China) according to the manufacturer’s instructions.

Techniques: In Vivo, Staining, Enzyme-linked Immunosorbent Assay, Protein Concentration

Journal: International Journal of Molecular Sciences

Article Title: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike Protein S1 Induces Methylglyoxal-Derived Hydroimidazolone/Receptor for Advanced Glycation End Products (MG-H1/RAGE) Activation to Promote Inflammation in Human Bronchial BEAS-2B Cells

doi: 10.3390/ijms241914868

Figure Lengend Snippet: SARS-CoV-2 S1 spike protein controls MG-H1/RAGE (hydroimidazolone/receptor for advanced glycation end products) proinflammatory pathway through Nrf2-dependent glyoxalase 1 (Glo1). Glo1 downregulation in human bronchial BEAS-2B cells. BEAS-2B cells were exposed to 25 ng/mL S1 for 24 h and ( a ) Glo1 mRNA, ( b ) protein expression, ( c ) specific activity, evaluated by real-time RT-PCR, Western blot (WB) and spectrophotometric assay, respectively, and ( d ) Nrf2 nuclear expression, evaluated by a specific assay, were studied. Pretreatment with 10 µM Nrf2-A (Nrf2 activator) rescued ( e ) Glo1 expression and ( f ) activity, reduced ( g ) MG-H1 intracellular accumulation, measured by a specific ELISA kit; ( h ) RAGE expression, evaluated by real-time RT-PCR, as well as inflammation, evaluated by IL-1β ( i ) and IL-6 levels ( j ), by real-time RT-PCR and ELISA, respectively, compared with S1-challenged cells. MG-H1 absolute levels (µg/mL) in order (mean ± SD): 0.54 ± 0.012, 0.83 ± 0.022, 0.529 ± 0.044, 0.527 ± 0.023. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) was used as internal loading control for WB normalization. Data represent mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Whole blots are shown in .

Article Snippet: Nrf2 Transcription Factor Assay kit (Colorimetric) (ab207223) (DBA Italia srl, Milan, Italy) was employed to detect Nrf2 activation in nuclear extracts, as per the manufacturer’s directions.

Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Spectrophotometric Assay, Enzyme-linked Immunosorbent Assay

Journal: Iranian Journal of Basic Medical Sciences

Article Title: p-Coumaric acid protects cardiac function against lipopolysaccharide-induced acute lung injury by attenuation of oxidative stress

doi: 10.22038/ijbms.2019.36316.8650

Figure Lengend Snippet: Effects of p-CA on LPS-induced systemic inflammation. Nrf2 mRNA expression in heart tissue analyzed. Data are expressed as the mean±SEM (n=8). * P <0.05, versus the Control rats. # P <0.05 versus the LPS group. Nrf2: Nuclear factor-erythroid 2 -related factor 2; LPS: Lipopolysaccharide; p-CA: p-Coumaric acid

Article Snippet: Expression of Nrf2 gene RNeasy plus mini kit (Qiagen Co, Netherlands) was used for RNA extraction.

Techniques: Expressing, Control